Audio: Targeted Re-Sequencing Made Easy: A Core Laboratory’s Experience with NimbleGen SeqCap EZ

Summary: From low throughput preparation of sample libraries to keeping the sequencing pipeline full, target enrichment and library preparation for next-generation sequencing has presented significant challenges at the Core Genotyping Facility at National Cancer Institute (NCI-CGF). Through key process optimization to library preparation coupled with the incorporation of NimbleGen SeqCap EZ, a solution-based enrichment technology, NCI-CGF has overcome the throughput challenges and increased capacity to manually enrich and process multiple 96-well plates. These improvements have proven to be significant, decreasing project turn-around times from one month to one week, opening the possibility of an automated solution, and decreasing costs while increasing efficiency for next generation targeted re-sequencing projects. Also presented in this webinar are target re-sequencing results from a genomic region associated with prostate cancer risk in 78 research samples. The results of this study have generated a detailed map of common genetic variations in chr19q13.33, and these variations should facilitate fine-mapping the association signal as well as determining the contribution of this locus to prostate cancer risk and regulation of prostate specific antigen (PSA) expression.